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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two added splice variants were described but in depth nucleotide sequence information will not be yet readily available.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This tutorial outlines even more achievable optimization of mobile and immunoassay parameters to make sure the best possible effects are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to ensure the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining through the blocked antibody as opposed to the antibody by yourself, one can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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